02512nas a2200229 4500008004100000022001400041245012000055210006900175260001200244300001000256490000800266520180100274100001902075700002402094700002302118700002002141700002002161700002202181700002002203700002302223856003602246 2020 eng d a1879-148400aInnate and adaptive immune cell subsets as risk factors for coronary heart disease in two population-based cohorts.0 aInnate and adaptive immune cell subsets as risk factors for coro c2020 05 a47-530 v3003 a

BACKGROUND AND AIMS: Cell-mediated immunity is implicated in atherosclerosis. We evaluated whether innate and adaptive immune cell subsets in peripheral blood are risk factors for coronary heart disease.

METHODS: A nested case-cohort study (n = 2155) was performed within the Multi-Ethnic Study of Atherosclerosis (MESA) and the Cardiovascular Health Study (CHS). Cases of incident myocardial infarction (MI) and incident angina (n = 880 total cases) were compared with a cohort random sample (n = 1275). Immune cell phenotypes (n = 34, including CD14 monocytes, natural killer cells, γδ T cells, CD4, CD8 and CD19 lymphocyte subsets) were measured from cryopreserved cells by flow cytometry. Cox proportional hazards models with adjustment for cardiovascular disease risk factors were used to evaluate associations of cell phenotypes with incident MI and a composite phenotype of incident MI or incident angina (MI-angina) over a median 9.3 years of follow-up. Th1, Th2, Th17, T regulatory (CD4CD25CD127), naive (CD4CD45RA), memory (CD4CD45RO), and CD4CD28 cells were specified as primary hypotheses. In secondary analyses, 27 additional cell phenotypes were investigated.

RESULTS: After correction for multiple testing, there were no statistically significant associations of CD4 naive, memory, CD28, or T helper cell subsets with MI or MI-angina in MESA, CHS, or combined-cohort meta analyses. Null associations were also observed for monocyte subsets, natural killer cells, γδ T cells, CD19 B cell and differentiated CD4 and CD8 cell subsets.

CONCLUSIONS: The proportions of peripheral blood monocyte and lymphocyte subsets are not strongly related to the future occurrence of MI or angina in adults free of autoimmune disease.

1 aOlson, Nels, C1 aSitlani, Colleen, M1 aDoyle, Margaret, F1 aHuber, Sally, A1 aLanday, Alan, L1 aTracy, Russell, P1 aPsaty, Bruce, M1 aDelaney, Joseph, A uhttps://chs-nhlbi.org/node/8403